Part:BBa_K2623011:Design

Phosphorylation pathsway builder of Kai

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 188
  Illegal BglII site found at 3666
  Illegal BglII site found at 3735
  Illegal BamHI site found at 2248
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used T4 DNA ligase to construct the circuit.

Source

This part contains the coding region of the Prochlorococcus (Synechococcus) elongatus PCC7942 CikA, RpaA and SasA gene.